Two micrograms of proteins were loaded and resolved on a 412% Bis-Tris Nupage (Invitrogen), transferred to PVDF membrane and the blot probed with anti-phosphotyrosine antibody (4G10, Upstate biotech)
Two micrograms of proteins were loaded and resolved on a 412% Bis-Tris Nupage (Invitrogen), transferred to PVDF membrane and the
