== Metabolic activity and viability of planktonic cells ofC. included XTT colorimetric assays Epibrassinolide for metabolic activity, CFU counts for viability, and microscopy for growth, viability, and morphological analysis. The CAGTA-enriched IgG portion significantly reduced the metabolic activity and viability ofC. albicanscompared to anti-blastospores. Purified CAGTA modified germ tube cell wall surfaces, as Epibrassinolide exposed by electron microscopy, and exhibited fungicidal properties by DiBAC fluorescent staining. In conclusion, antibodies in response to invasive candidiasis have antifungal activity againstCandida albicans, influencing metabolic activity, viability, and cell wall structure, leading to cell death. These findings suggest the potential energy of CAGTA as diagnostic markers and support the possibility of developing immunization protocols againstCandidainfections. Keywords:Candida albicans, Invasive candidiasis, Antibodies, Growth inhibitor, Fungicides == Intro == Invasive Candidiasis (IC) is definitely a fungal illness caused by yeasts of the genusCandida, among whichCandida albicansis the most frequently isolated, affecting more than 250,000 people worldwide every year and cause more than 50,000 deaths [1]. Some varieties ofCandidaare common colonizers of the skin, and of the gastrointestinal, genitourinary and respiratory tract Epibrassinolide mucous membranes. Nevertheless, under particular circumstances, they can cause both superficial and invasive infections, and the second option are associated with high mortality rates due to the diagnostic problems derived from the absence of pathognomonic indications [2]. Despite some improvement in diagnostic methods and the intro of new medicines for treatment, IC is still a major problem in critically ill individuals [35]. Other risk conditions for an invasive fungal disease (IFD) are immunosuppression, HIV infected individuals, recipients of hematopoietic or solid organ transplantation, and long stay in the ICU [6,7]. Globally, the incidence ofC. albicansisolates is definitely reducing (65.3% to 48.4%), in favor of non-albicans Candidaspecies (NAC) such asCandida glabrata(from 11.3 Epibrassinolide to 18.2%),Candida parapsilosis(6% to 17.1%) orCandida tropicalis(7.2% to 10. 6%) [8]. In Spain, today,C. albicansis still the major cause of IC (47.7%), followed byC. parapsilosis(22%),C. glabrata(15.8%),C. tropicalis(9.3%) andCandida krusei(2.8%) [9]. Blood culture remains the gold standard for analysis of IC or, on the other hand, histopathological evidence in normally sterile sites, but both techniques have low level of sensitivity, and a long turnaround time (7296 h) leading to a delay in specific treatment that results in improved mortality [10]. In this line, a multicenter retrospective study carried out in 23 ICUs of 9 European countries reported an IC incidence of 7.07 episodes per 1000 admissions having a 30-day time mortality of 42% [11]. In an attempt to improve the diagnostic resources for IC, our group of study developed an indirect immunofluorescence (IIF) assay detecting antibodies that recognize specific antigens located on the cell wall surface ofC. albicansgerm tubes; such antibodies are connected to the invasive candidiasis process and were termed CAGTA (Candida albicansGerm Tube Antibodies) [1218]. The detection of CAGTA offers achieved very interesting results, actually in neutropenic nicein-125kDa individuals, and its diagnostic value has been proved successful for monitoring antifungal therapies [19]. A Spanish multicenter study with 53 IC individuals admitted to ICU evidenced that those with improved CAGTA titers along with antifungal treatment greatly reduced their connected mortality rate from 61.2 to 22.7% [20]. This element is related to an efficient anti-Candidaresponse that requires the assistance of different mechanisms of the immune system [2124]. In this regard, antibodies confer safety against fungal infections by opsonization and phagocytosis, match activation and antibody-dependent cell toxicity [25,26]. In addition to direct neutralization of fungi and their antigens, antibodies can inhibit fungal growth, modify gene manifestation, signaling and lipid metabolism, induce iron starvation, and reduce polysaccharide launch and biofilm formation [6,24]. Furthermore, the recognition of specific antigens that are identified by CAGTA, such as hyphal wall protein 1 (Hwp1), agglutinin-like sequence protein 3 (Als3), and methionine synthase protein 6 (Met6) offers served to develop experimental immunoassays to detect antibodies that may help to the analysis of IC in individuals at risk, actually in immunocompromised ones [27]. In this collection, since CAGTA have been related to a better prognosis of the illness, users of our group have assayed some epitopes of the antigens identified by CAGTA like a complex peptide vaccine conjugated to KLH (3P-KLH) for safety inside a murine model of hematogenously disseminated candidiasis, with encouraging results (Diez et al. data not published). In fact, individuals who survived systemic infections developed strong antibody reactions to certainC. albicansproteins that could help like a prognostic signature for an IC clinical-outcome prediction model [2830]..